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Hsd11b2 catalyzes the conversion of cortisol to cortisone (Fuller et al., 2012), and also plays a crucial role in the conversion of 11β-OH-androstenedione to 11-ketotestosterone (11-KT) (Tokarz et al., 2015), which affects the production of the most important androgen in teleost fish. Furthermore Hsd17b1 converts inactive estrone to active estradiol (Mindnich et al., 2004), Hsd17b3 is an essential enzyme for the synthesis of 11-KT (Mindnich et al., 2005) and Hsd20b2 converts 17alpha-hydroxyprogesterone to 17alpha,20beta-DP, which has been identified as the maturation-inducing hormone of several teleosts (Senthilkumaran et al., 2002). Gene Expression Profiling by Quantitative Real-Time PCR (qRT-PCR) Figure 4. Clustering of the expression profiles of 19 grouper β -hsd genes during sex reversal. Genes were clustered according to phylogenetic relationships in expression profiles. The RPKM values were transformed into Z scores. Z scores were plotted according to Z = (x - μ)/σ, where x is the log 2 transformed gene expression measurement and μ and σ are the mean and standard deviations of expression of the gene. Red and blue colors indicate high and low relative expression levels after homogenization, respectively.
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