Little is known about the corresponding grouper genes; however, the availability of the complete grouper genome sequence has made it possible to identify all the β -hsd genes in this species for the first time. In the present study, we systematically idntified 19 putative β -hsd genes in the E. coioides genome, and subsequently characterized the genes in terms of position in the genome, exon-intron structure and conserved domains. In addition, we also isolated β -hsd genes in samples of human, mouse, chicken, xenopus, coelacanth, spotted gar, zebrafish, fugu, tilapia, medaka, stickleback, and common carp. Chain – casino esc several single bets on irrelevant outcomes. Our study represented the first comprehensive investigation of the fish β -hsd gene family, and the resulting data will undoubtedly be useful for future analyses, including further research into the role of β -hsd genes and sex steroids in sex reversal of grouper. The orange-spotted grouper is a typical protogynous hermaphrodite species. Previous studies have shown that groupers in the natural environment generally develop into female individuals during the first sex differentiation (Liu and de Mitcheson, 2009).
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