These results are consistent with the more rapid transport of estrogen sulfates than of glucuronides from amniotic fluid. About the same time, Warren and French (1965) showed that homogenates of unseparated human fetal membranes actively hydrolyze DHEAS. In an extensive study of whole tissue fragments, Tseng et al. (1972) demonstrated that human amnion and chorion contain estrogen sulfatase and 17 β -dehydrogenase activities, whereas the chorion, but not the amnion, contain low levels of aromatase activity. Gant et al. (1977) carefully examined the biochemical properties of sulfatase activity with both DHEAS and P 5 S as substrates. Sulfatase activity was found in the microsomal fraction prepared from chorion but not amnion.
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