Gene Expression Profiling by Quantitative Real-Time PCR (qRT-PCR) Figure 4. Clustering of the expression profiles of 19 grouper β -hsd genes during sex reversal. Genes were clustered according to phylogenetic relationships in expression profiles. The RPKM values were transformed into Z scores. Z scores were plotted according to Z = (x - μ)/σ, where x is the log 2 transformed gene expression measurement and μ and σ are the mean and standard deviations of expression of the gene. Red and blue colors indicate high and low relative expression levels after homogenization, respectively. The RPKM value for each gene and each stage was provided in the figure.
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