Gene Expression Profiling by Quantitative Real-Time PCR (qRT-PCR) The exon-intron structure of the grouper β -hsd genes was further characterized, and showed that the number of introns in each β -hsd gene varied from 2–16 (Figure 2B). Hsd3b1 was the gene with the lowest number of introns, containing only two, while hsd17b4 had the greatest amount at 16. In addition, a phylogenetic tree was constructed using the grouper β-HSD protein sequences to determine whether the exon-intron structure is consistent with the phylogenetic tree (Figure 2A). PearFiction tenis all star 33 Studios. However, due to large number of exon/intron structure types of the grouper β -hsd genes, only a few genes shared similar structures. Figure 4.
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